FastQCFastQC Report
lun 30 set 2024
L4_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameL4_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28010533
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAATTGAACTTTATCCACAAAAAAGTACTGAGAAGGAAAGTCTTGCC754540.2693772374841993No Hit
GTAGAAACTAACCTGGCTTACGCCGGTCTGAACTCAGATCACGTAGGGGT665730.23767130743281464No Hit
GCTAGATTCAATTAATCAACTTTAAATTATTATTTAAACGACCCTGGTTA575360.20540844403067948No Hit
GCGGAGGCAAGTTTTGCCGAATTCCTTCCAAAATTTTTGGAATAGATTCT527730.18840412640487775No Hit
GTTTAATAGTCGAACAGACTACCTGATTAAGCTGCTGCACCAAATAGGGT452720.16162491445628685No Hit
GCAGGCTCGACCTCCTAAAGTTAGGAGGCGATGTTTTTGTTAAACAGGCG418880.1495437448476971No Hit
GGAAAGTCTTGCCAATTTTAAAGAATTTATTTATTATACCTTTTGTATCA414990.14815498155640236No Hit
CTCGAGTGTTTGTTCTCTCCTCGACGTCGCCCCAACCAAACATCCTCCTC387430.13831582569314194No Hit
GTTTGGTTGGGGCGACGTCGAGGAGAGAACAAACACTCGAGAATATACAT337550.12050823881144997No Hit
GTTTGTTCTCTCCTCGACGTCGCCCCAACCAAACATCCTCCTCTTCCACC323940.11564935233470924No Hit
GAAAGTCTTGCCAATTTTAAAGAATTTATTTATTATACCTTTTGTATCAG315090.11248982659487416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)