FastQCFastQC Report
mar 1 ott 2024
L4_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameL4_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28010533
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAATTGAACTTTATCCACAAAAAAGTACTGAGAAGGAAAGTCTTGCC792030.2827614883301221No Hit
GCTAGATTCAATTAATCAACTTTAAATTATTATTTAAACGACCCTGGTTA576110.20567620044930954No Hit
GTAGAAACTAACCTGGCTTACGCCGGTCTGAACTCAGATCACGTAGGGGT565560.2019097601605796No Hit
GCGGAGGCAAGTTTTGCCGAATTCCTTCCAAAATTTTTGGAATAGATTCT468220.16715854710797542No Hit
GCAGGCTCGACCTCCTAAAGTTAGGAGGCGATGTTTTTGTTAAACAGGCG440760.1573550921005323No Hit
GGAAAGTCTTGCCAATTTTAAAGAATTTATTTATTATACCTTTTGTATCA436080.15568429204828055No Hit
GTTTAATAGTCGAACAGACTACCTGATTAAGCTGCTGCACCAAATAGGGT430650.1537457355773987No Hit
GTTTGGTTGGGGCGACGTCGAGGAGAGAACAAACACTCGAGAATATACAT420180.1500078559733226No Hit
GAAAGTCTTGCCAATTTTAAAGAATTTATTTATTATACCTTTTGTATCAG318130.11357513261172146No Hit
GTTTGTTCTCTCCTCGACGTCGCCCCAACCAAACATCCTCCTCTTCCACC290080.10356104255495602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)